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Maximum likelihood tree (Tamura-Nei model) of all Brettanomyces strains investigated in this study, based on partial large-subunit rRNA gene sequences (457 to 471 bp). Bootstrap values of >80% (based on 1,000 replicates) are given at the nodes of the tree. Saccharomyces cerevisiae was used as an outgroup. The origins of the different strains, i.e., beer, Cantillon Brewery, soft drinks, others, and wine, are highlighted in green, dark green, orange, blue, and red, respectively.
