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. 2014 Jul;80(14):4398–4413. doi: 10.1128/AEM.00601-14

TABLE 1.

Brettanomyces isolates used in this study

Species Isolatea Isolateb LSU rRNA gene GenBank sequence accession no. Geographic origin Yr of isolation Niche Clusterc Subclusterd
B. anomalus ST05.12/06 CBS 5111 KF790764 Ireland —e Beer I I-A
ST05.12/15 MUCL 27703 KF790805 — 1909 Beer I I-B
ST05.12/13 MUCL 27702 KF790807 United Kingdom 1940 Bottled beer I I-B
ST05.12/19 MUCL 31218 KF790801 United Kingdom — Bottled beer I I-B
ST05.12/09 CBS 4460 KF790763 — — Cider I I-B
ST05.12/35 NRRL Y17520 KF790784 — — Cider I I-B
ST05.12/61 CBS 4210 KF790767 — — Cider I I-B
ST05.12/17 MUCL 49367 KF790803 The Netherlands — Soft drink I I-B
ST05.12/39 NRRL Y17522 KF790781 The Netherlands — Soft drink I I-B
B. bruxellensis ST05.12/18 MUCL 27705 KF790802 South Africa — Bantu beer II II-A
ST05.12/26 MUCL 49865 KF790794 Belgium — Brewery II II-B
ST05.12/48f,h KF790778 Belgium 2012 Cantillon Brewery II II-B
ST05.12/49g,i KF790777 Belgium 2012 Cantillon Brewery II II-D
ST05.12/50g,i — Belgium 2012 Cantillon Brewery II II-D
ST05.12/51g,j KF790775 Belgium 2012 Cantillon Brewery II II-D
ST05.12/52g,j KF790774 Belgium 2012 Cantillon Brewery II II-D
ST05.12/53h,k KF790773 Belgium 2012 Cantillon Brewery II II-D
ST05.12/16 MUCL 27701 KF790804 United States — Dry ginger ale II II-A
ST05.12/59 CBS 6055 KF790768 United States — Dry ginger ale II II-A
ST05.12/54 CBS 73 KF790772 France — Grape must II II-C
ST05.12/22 VIB X9085 KF790798 Belgium — Lambic beer II II-B
ST05.12/24 MUCL 27707 KF790796 Belgium — Lambic beer II II-B
ST05.12/25 MUCL 27700 KF790795 Belgium — Lambic beer II II-B
ST05.12/27 MUCL 30490 KF790793 Belgium 1989 Lambic beer II II-B
ST05.12/40 MUCL 30489 KF790779 Belgium 1989 Lambic beer II II-B
ST05.12/36 NRRL Y1413 KF790783 Belgium — Lambic beer sediment II II-B
ST05.12/23 KaHoSL 01 KF790797 Belgium — Lambic beer II II-B
ST05.12/28 KaHoSL 02 KF790792 Belgium — Lambic beer II II-B
ST05.12/55 CBS 3025 KF790771 United Kingdom — Secondary beer fermentation II II-B
ST05.12/21 MUCL 27706 KF790799 The Netherlands — Soft drink II II-A
ST05.12/30 CBS 8027 KF790789 The Netherlands — Soft drink II II-A
ST05.12/33 CBS 98 KF790786 The Netherlands 1939 Stout II II-B
ST05.12/34 CBS 97 KF790785 United Kingdom 1939 Stout II II-B
ST05.12/56 CBS 2499 KF790770 France — Wine II II-B
ST05.12/62 AWRI 1499 KF790766 Australia — Wine II II-E
B. custersianus ST05.12/04 CBS 5207 KF790780 South Africa — Bantu beer III III-A
ST05.12/05 CBS 5208 KF790776 South Africa — Bantu beer III III-A
ST05.12/11 CBS 4806 KF790809 South Africa — Bantu beer III III-B
ST05.12/12 MUCL 27704 KF790808 South Africa 1960 Bantu beer III III-B
ST05.12/29 CBS 8347 KF790791 The Netherlands 1996 Olives III III-C
B. naardenensis ST05.12/10 CBS 6043 KF790810 The Netherlands — Carbonated tonic water IV IV-A
ST05.12/01 CBS 6042 KF790811 The Netherlands — Lemonade IV IV-A
ST05.12/02 CBS 6107 KF790800 The Netherlands — Lemonade IV IV-A
ST05.12/03 CBS 6115 KF790790 The Netherlands — Soft drink IV IV-A
ST05.12/07 CBS 7540 KF790765 South Africa — Soft drink IV IV-B
ST05.12/14 MUCL 27708 KF790806 The Netherlands — Soft drink IV IV-A
ST05.12/37 NRRL Y5740 KF790782 United States — Soft drink IV IV-A
ST05.12/57 CBS 6040 KF790769 United States — Soda water IV IV-A
B. nanus ST05.12/31 CBS 1956 KF790788 Sweden — Bottled beer V V-A
ST05.12/32 CBS 1955 KF790787 Sweden — Bottled beer V V-A
a

Our own isolate numbering.

b

AWRI, Australian Wine Research Institute, Glen Osmond, Australia; CBS, Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands; NRRL, Agriculture Research Service Culture Collection, National Center for Agricultural Utilization Research, Peoria, IL; KaHoSL, Katholieke Hogeschool Sint-Lieven, Ghent, Belgium; MUCL, Mycothèque de l'Université Catholique de Louvain, Louvain-la-Neuve, Belgium; VIB, Vlaams Instituut voor Biotechnologie, Leuven, Belgium.

c

Clustering results (DNA fingerprinting results for combined data sets) at a similarity percentage of 66%, perfectly corresponding to species delineation.

d

Clustering results (DNA fingerprinting results for combined data sets) at a similarity percentage of 80%.

e

—, unknown.

f

Sampled (February 2012) from a 50-year-old cask; the beer was brewed on 13 November 2011.

g

Sampled (February 2012) from a 6- to 7-year-old cask; the beer was brewed on 12 December 2011.

h

Sampled (February 2012) from an 8-year-old cask; the beer was brewed on 12 December 2011.

i

Isolated using Wallerstein laboratory nutrient (WLN) agar.

j

Isolated using universal beer agar (UBA).

k

Isolated using Dekkera/Brettanomyces differentiation medium (DBDM).