. 2014 Jul;80(14):4215–4225. doi: 10.1128/AEM.00290-14

TABLE 2.

Primers used in this study

Use and published name	Plasmid or probe(s)	Sequence (5′–3′)
For construction of the GlxR expression plasmid
glxre01	pZWHJ024	GGAATTCCATATGGAAGGTGTACAGGAGATCCTG
glxre02	pZWHJ024	CCGGAATTCTTATCGAGCGCGACGTGCC
glxre08	pZWHJ033	ACGCGTCGACAAAGGAGGACAACCAAAGCAGTGGAAGGTGTACAGGAGATCCTG
glxre09	pZWHJ033	CCGGAATTCTTATCGAGCGCGACGTGCC
For mutated GlxR binding sites
pDFMxmut01	DFMax	GTGACGTGGAACATATTGGGCTTTGAAAGGTGACTC
pDFMxmut02	DFMax	TATGTTCCACGTCACAAAGTGTTCAAGGGCTAGACGTATGC
pR-KHxmut01	R-KHax01	CACAGCCAAGTTGTGATCAAAGGGATTCCGCTATCTG
pR-KHxmut02	R-KHax01	CACAACTTGGCTGTGCTCGATTATGTGTTTATGAC
pR-KHxmut03	R-KHax02	CCGAGACACGACTCTGTGTGAAGTCCTCCCGATTCTGGTGCG
pR-KHxmut04	R-KHax02	AGAGTCGTGTCTCGGAGCATCGAATAGCTCCCAAAC
For EMSA of His₆-GlxR
pgenDFM04	DFMan and DFMaxm	CCCAAGCTTGCGCCCATTGTTGGAGTCC
pgenDFM09	DFMan and DFMaxm	CGCGGATCCATGGGGGGAATTTTCAGAGCTG
pgenR04	R-KHan	CCCAAGCTTGGCGACGTTATCCATAATC
pgenKH07	R-KHan	CCCAAGCTTGCCAATGAGCACCGCAGCCAAG
pgenR02	R-KHax01 and R-KHax01 m	CCCAAGCTTGCACCGGAAATGGTCAAAC
pgenR03	R-KHax01 and R-KHax01 m	CGCGGATCCTCTGGTGCGTGTGATGTC
pgenKH11	R-KHax02 and R-KHax02 m	CGCGGATCCTATGACATCACACGCACCAG
pgenKH09	R-KHax02 and R-KHax02 m	CCCAAGCTTAAGTTCCACTCCTTAGCCAG