TABLE 1.
Sequence information summary for the RNA samples extracted from the Cartwright culture during growth on benzoate and benzene using pyrosequencing or Illumina sequencing
| Sequencing method and parameter | Benzene RNA | Benzoate RNA |
|---|---|---|
| Pyrosequencing (avg read length, 160 bp) | ||
| Total reads | 196,075 | 250,500 |
| Non-rRNA reads (% of total reads) | 2,153 (1.1) | 4,970 (2) |
| Non-rRNA contigs/isotigs | 81 | 219 |
| Annotated mRNA contigs (BLASTx) | 75 | 189 |
| Annotated mRNA singletons (BLASTx) | 490 | 1,831 |
| Illumina sequencing (read length, 75 bp) | ||
| Total reads | 26,893,212 | 28,567,030 |
| Non-rRNA reads (%) | 849,441 (3) | 1,149,965 (4) |
| Non-rRNA contigs | 366,368a | |
| Contigs that passed the abundance thresholdb | 37,353a | |
| Annotated mRNA contigs (BLASTx) | 14,038a |
a
The number shown is the number of contigs assembled from pooled benzene and benzoate RNA reads.
b
The abundance threshold is a contig length greater than 100 b and average coverage greater than 3.