TABLE 3.
Characteristics of primer pairs used in this study
| Primer paira | Sequence (5′ to 3′)b | Positionsc | Major gene targeted | Expected amplicon size (bp) | Annealing temp (°C) |
|---|---|---|---|---|---|
| Tect1 (f) | GAAGGTGTWTGGACRTTHCC | 3162–4442 | DNA polB | 1,280 | 56 |
| Tect2 (r) | ACYHGCCATDTCYCGCAC | ||||
| Tect3 (f) | CCGGYGGWWTRTCMGGDCTTG | 11096–11838 | mur1 | 742 | 59 |
| Tect4 (r) | AAGTCCATHGCCATGCCTTGG |
a
f and r, forward and reverse primers, respectively.
b
Key to symbols: W, A + T; R, A + G; H, A + T + C; Y, C + T; D, G + A + T; M, A + C.
c
Positions correspond to the primer annealing sites in the GIL01 genome.