TABLE 4.
Effect of rhizobial IAA on Medicago sativa growth and nitrogen fixation
| Plant | Shoot fresh wt (mg)a | Secondary root no.b | Nodule no.b | Relative levelc |
|---|---|---|---|---|
| Ms-1021 | 231 ± 51 a | 15 ± 4 a | 9 ± 3 a | |
| Ms-RD64 | 303 ± 83 b | 17 ± 2 a | 11 ± 3 a | 1.5 ± 0.1 |
| Ms-RD65 | 381 ± 87 c | 19 ± 2 b | 14 ± 3 b | 2.9 ± 0.3 |
| Ms-RD66 | 414 ± 81 c | 19 ± 2 b | 18 ± 4 c | 3.3 ± 0.5 |
| Ms-RD67 | 311 ± 70 b | 15 ± 3 a | 13 ± 4 a | 1.8 ± 0.2 |
Data represent means ± SD (n = 50), and those marked with different letters are significantly different based on the Tukey's test (P < 0.01).
Data were obtained from the analysis of equal root portions and represent means ± SD (n = 15); those marked with different letters are significantly different based on the Tukey's test (P < 0.01).
Relative gene expression levels of nifH gene from comparative CT method; 2−ΔΔCT > 1, more highly expressed genes in plants nodulated by the modified strains (RD64, RD65, RD66, and RD67); 2−ΔΔCT < 1, more highly expressed genes in plants nodulated by the wild-type strain 1021. Values represent means ± SD of the results of four biological replicates conducted at different times.