FIG 4.

Different CDR insertions in loop 9. (A and B) Construction of different CDR insertions. (A) Selected light-chain CDRs and three CDR sequences are indicated. (B) Selected heavy-chain CDRs and three CDR sequences are indicated. (C) SDS-PAGE assay of the purified target proteins. Lane M, midrange protein molecular mass markers; lane 1, G9-L1; lane 2, G9-L2; lane 3, G9-L3; lane 4, G9-H1; lane 5, G9-H2; lane 6, G9-L3. (D) ELISA. All ELISA steps were performed as described in Materials and Methods.