TABLE 3.

Primers used for Lys scanning in LCDR3 and HCDR3

Target gene and primer	DNA sequence (5′–3′)
LCDR3-Lys
L-Q/K-F	GATGGATCCCACAAGTATCATCGTTCCCCACGGAC
L-Y/K-F	GATGGATCCCACCAGAAACATCGTTCCCCACGGAC
L-H2/K-F	GATGGATCCCACCAGTATAAACGTTCCCCACGGAC
L-R1/K-F	GATGGATCCCACCAGTATCATAAATCCCCACGGAC
L-S/K-F	GATGGATCCCACCAGTATCATCGTAAACCACGGAC
L-P/K-F	GATGGATCCCACCAGTATCATCGTTCCAAACGGAC
L-R2/K-F	GATGGATCCCACCAGTATCATCGTTCCCCAAAGAC
HCDR3-Lys
H-F/K-F	ATTGGATCCAAACCCCATCTCGAGGACTACTGGTACTTCGATC
H-P/K-F	ATTGGATCCTTCAAACATCTCGAGGACTACTGGTACTTCGATC
H-H/K-F	ATTGGATCCTTCCCCAAACTCGAGGACTACTGGTACTTCGATC
H-L/K-F	ATTGGATCCTTCCCCCATAAAGAGGACTACTGGTACTTCGATC
H-E/K-F	ATTGGATCCTTCCCCCATCTCAAAGACTACTGGTACTTCGATC
H-D1/K-F	ATTGGATCCTTCCCCCATCTCGAGAAATACTGGTACTTCGATC
H-Y/K-F	ATTGGATCCTTCCCCCATCTCGAGGACAAATGGTACTTCGATC
H-W/K-F	ATTGGATCCTTCCCCCATCTCGAGGACTACAAATACTTCGATC
H-Y2/K-F	ATTGGATCCTTCCCCCATCTCGAGGACTACTGGAAATTCGATC
H-F/K-F	ATTGGATCCTTCCCCCATCTCGAGGACTACTGGTACAAAGATC
H-D2/K-F	ATTGGATCCTTCCCCCATCTCGAGGACTACTGGTACTTCAAAC