Figure 2.

Knockdown of KNDC1 promotes cell proliferation and partially reverses cellular senescence in aging HUVECs. Following transfection with NT-siRNA or KNDC1-siRNA, the expression levels of KNDC1 mRNA and protein were determined by (A) quantitative polymerase chain reaction and (B) western blotting. The percentage of senescent HUVECs transfected with (C) NT-siRNA or (D) KNDC1-siRNA was determined by senescence-associated β-galactosidase staining (n=3; magnification, ×200). Values are presented as the mean ± standard deviation. ^*P<0.05 vs NT-siRNA. KNDC1, kinase noncatalytic C-lobe domain containing 1; HUVECs, human umbilical vein endothelial cells; NT-siRNA, non-targeting control pool; siRNA, small interfering RNA.