Figure 3.

Changes in cell cycle progression and capillary tube network formation following knockdown of KNDC1. Following transfection with (A) KNDC1-siRNA or (B) NT-siRNA, the cell cycle was analyzed by flow cytometry with propidium iodide staining. HUVECs transfected with (C) KNDC1-siRNA or (D) NT-siRNA were seeded on Matrigel-coated 96-well plates (3×10⁴ cells/well) in 100 μl M199 medium. Following incubation for 24 h, the mean tubule length and the number of branch points were calculated under an Olympus inverted microscope (magnification, ×40). KNDC1, kinase noncatalytic C-lobe domain containing 1; HUVECs, human umbilical vein endothelial cells; NT-siRNA, non-targeting control pool; siRNA, small interfering RNA.