Figure 2.

Alt repressed RV-induced IFN expression and enhanced RV replication in epithelial cells. Cells were infected with RV 16 and treated with Alt individually or in combination. RNA was collected 24 hours later and was analyzed by real-time PCR. Fold induction of IFN-β and IFN-λ1 (A) or relative abundance of viral RNA (vRNA) (B) were determined. *P < 0.05 (n = 5) when the treated samples (Alt, RV, or Alt+RV) were compared with the control. ^#P < 0.05 (n = 5) when the samples with cotreatment (RV+Alt) were compared with RV-infected samples. ^$P < 0.05 (n = 5) when RV-infected samples were compared with the cotreatment (RV+Alt) samples. (C) Cellular protein was collected and analyzed by Western blot. VP0 and VP2 = RV coat protein. Actin was used as a loading control. The image is representative of at least three independent experiments.