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. 2014 Jun;50(6):1136–1143. doi: 10.1165/rcmb.2013-0357OC

Figure 7.

Figure 7.

Separate fungal components are involved in the effects of Alt on virus-induced epithelial immunity. The cells were treated with ds (25 μg/ml) or Alt (21.5 μg/ml) individually or in combination for 3 hours, and RNA samples were analyzed by real-time PCR analysis. (A, B) Alt effects were heat sensitive. H-Alt = heat-inactivated Alt. #,$P < 0.05 (n = 5) when Alt-treated samples were compared with H-Alt–treated samples. (C) Alt’s effect on IL-6 expression was protease dependent. Alt was preincubated with protease inhibitor mix (PI) for 30 minutes before the treatments. The same percentage of DMSO (solvent for PI) was added to non-PI samples as the negative control. #P < 0.05 (n = 5). (D) Alt’s effect on IFN-β was protease independent.