Figure 2.
Histological Evaluation of the influence of nmMLCK expression on lung inflammation and airway remodeling. Histology (hematoxylin and eosin [H&E]) sections in WT, nmMLCK−/−, and nmMLCKec/ec mice demonstrating leukocyte infiltration and recruitment to the (A) peribronchial and (B) perivascular areas, respectively, after OVA challenge. (C) Lung sections stained with periodic acid-Schiff (PAS) to detect goblet cell (arrows) presence and activity in PBS or OVA-challenged mice. (D) Major basic protein (MBP) staining in tissue microarray samples derived from the OVA-challenged murine lines. All histological evaluations were quantified and summarized in the bar graphs accompanying each analysis shown in the last column. *P < 0.05; n = 5–6. OD, optical density.