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Relaxation to RGZ, but not ALB, is maintained with increasing MCh contraction. Airways in lung slices were precontracted with 30, 300, and 3,000 nM MCh before perfusion with 100 μM RGZ (n = 4) or 10 μM ALB (n = 4). Representative frame-by-frame analysis (0.5 Hz) of changes in lumen area with time in the presence of each concentration of MCh alone and with RGZ (A) or ALB (C). Responses (mean ± SEM) to RGZ (C) or ALB (D) are expressed as % relaxation of the sequential precontractions.
