Figure 4.

RGZ elicits relaxation of mouse small airways independently of peroxisome proliferator activated receptor (PPAR)γ activation. Airways in lung slices were precontracted with various constrictors before perfusion with PPARγ agonists including RGZ. Responses to RGZ after precontraction with 300 nM MCh (n = 4), 10 nM Et-1 (n = 4), or 100 nM 5HT (n = 4) (A); RGZ, troglitazone (TGZ), and ciglitazone (CGZ) after precontraction with 300 nM MCh (all n = 4) (B); and RGZ in the absence and presence of 10 μM GW9662 (PPARγ antagonist) after precontraction with 300 nM MCh (n = 9 and n = 5, respectively) (C). Responses (mean ± SEM) are expressed as % initial airway lumen area after precontraction with MCh, endothelin (Et)-1, or 5HT (A, left panel) or as % relaxation of the submaximal precontraction (A [right panel], B, C).