Figure 5.

RGZ inhibits MCh-induced calcium oscillations in airway smooth muscle cells (ASMCs) in mouse small airways. Ca²⁺ oscillations were measured in ASMCs within airways in lung slices incubated with 20 μM Oregon Green-AM. (A) Representative trace showing Ca²⁺ oscillations induced by 300 nM MCh and the inhibitory effect of 100 μM RGZ. (B) Ca²⁺ oscillation frequency of ASMCs after 4 minutes of RGZ exposure. Responses (mean ± SEM) are expressed as % of the Ca²⁺ oscillation frequency in the presence of MCh in four to eight slices from four mice. *P < 0.05 one-way ANOVA, methacholine precontraction alone. F_t/F₀ = ratio of final fluorescence normalized to the initial fluorescence.