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. 2014 Mar;50(3):549–558. doi: 10.1165/rcmb.2013-0228OC

Figure 1.

Figure 1.

Dose-dependent effects of whole cigarette smoke (WCS) on ion transport function. Well-differentiated non–cystic fibrosis (CF) primary human bronchial epithelial (HBE) cells were exposed to varying amounts of WCS or air control and then studied in Ussing chambers under short-circuit current (Isc) conditions. (A) Representative Isc tracing of HBE cells exposed to WCS for 0, 10, 20, or 30 minutes. The experiment included serial additions of amiloride (100 μM), chloride secretory gradient with amiloride, the cyclic adenosine monophosphate (cAMP) agonist forskolin (20 μM), and CFTRInh-172 (10 μM) to confirm CF transmembrane conductance regulator (CFTR) dependence. (B) Forskolin-stimulated change in Isc is shown for each cigarette smoke exposure level. *P < 0.05; **P < 0.005 (n = 4 per concentration). (C) Transepithelial resistance (TER) of HBE monolayers at the start of the experiment. **P < 0.005; *P < 0.05 (n = 4).