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Effect of roflumilast and WCS exposure on airway surface liquid (ASL) depth. (A) Representative confocal Z-scan image of well-differentiated non-CF HBE cells exposed to air control or to WCS (10 min) and treated with roflumilast (30 nM) or vehicle (Veh.) to the basolateral compartment for 24 hours before the assay. (B) Summary data of ASL depth across experiments. *P < 0.05 (n = 6 monolayers per condition).
