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. 2014 Mar;50(3):549–558. doi: 10.1165/rcmb.2013-0228OC

Figure 6.

Figure 6.

The effect of roflumilast on CFTR-mediated intestinal fluid secretion. (A) Representative Isc tracing of differentiated T84 intestinal epithelial cells studied in Ussing chambers under Isc conditions. The experiment included serial additions of amiloride (100 μM), chloride secretory gradient with amiloride, roflumilast (30 nM), or vehicle control followed by CFTRInh-172 (10 μM). (B) Summary data of change in stimulated Isc as shown in A. *P < 0.05 (n = 6 per condition). (C) Representative photograph of ligated CFTR+/+ mouse intestine after 3 hours of incubation with roflumilast (30 nM) or vehicle control in DMEM vigorously gassed with 95% O2/5% CO2 and held at 37°C. The scale bar is 1 cm. (D) Summary data of relative wet/dry ratio of murine intestine CFTR+/+ and CFTR−/− mice after 3 hours of incubation with test compounds. Forskolin control was 20 μM exposure. **P < 0.005 (n = 6 per condition). (E) Change in diameter of ligated intestine after 3 hours of incubation as detected by investigator blinded to treatment assignment. *P < 0.05 (n = 6 per condition).