Figure 1.

Smoke exposure induces c-Src and c-Raf phosphorylation in mouse lungs. c-Src activity (A) and gene expression (B) were measured in the lungs of wild-type nonexposed mice and various times of smoke-exposed mice (n = 5 for each group). (C) c-Src activity correlated with gene expression. (D) Immunoblots and densitometry analysis were performed for p-Src(Tyr416), total c-Src, p-Raf(ser388), total c-Raf, and actin on protein from the same samples in A. (E) Immunofluorescence analysis was performed for p-Src(Tyr416) and p-Raf(ser388) on tissue from room air–exposed and 1-year cigarette smoke–exposed mice (bar = 50 μM for the upper panels, and bar = 20 μM for the lower panels under higher magnification). Isotype control rabbit IgG were used as negative controls in each assay (bottom panel).