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. 2014 Mar;50(3):559–570. doi: 10.1165/rcmb.2013-0258OC

Figure 3.

Figure 3.

Cigarette smoke–activated epidermal growth factor receptor (EGFR) is regulated by c-Src to induce MAPK activation. (A) Immunoblots for c-Src, c-Raf ,and MAPK phosphorylation were conducted on cell lysate protein from SAE cells transfected with negative control and EGFR siRNA and then treated with CSE (90 min). (B) c-Src activity assays were conducted on control or 24-hour CSE-treated cells that have be transfected with control and EGFR siRNA (n = 7). (C) SAE cells were treated with CSE over multiple time points with and without AZD-0530 treatment, and phosphorylation of EGFR at Tyr1068 was examined by immunoblots. (D) EGFR tyrosine phosphorylation was examined after transfection with negative control and c-Src siRNA and treatment with 10 ng/ml epidermal growth factor or 5% CSE (0, 15, 30, 60, 90, or 120 min).