Figure 5.

Gene expression of (a) NADPH oxidase enzymes in cultured ST2 cells as measured by real-time PCR, normalized to GAPDH mRNA; fold expression is reported as mean ± SEM. In a separate experiment, (b) ST2 cells were pre-incubated with different NADPH oxidase inhibitors for 30 minutes prior to EtOH-treatment (50 mM) for 1 hr at 37°C, at which H₂0₂ production was measured using the Amplex Red hydrogen peroxide/peroxidase assay kit as described in Materials and Methods. Data presented here are representative of two independent experiments performed in triplicate and expressed as the rate of H₂0₂ production per minute which were corrected for background absorbance by subtracting experimental values from those obtained in control wells; ST2 cells +/- inhibitor without EtOH treatment. Statistical significance was determined by One-way ANOVA followed by Student-Newman Keuls post hoc analysis, a<b, p<0.05.