Table 1.
Species-specific ITS rDNA primers
| Species [GenBank Acc.Number] | Annealing temperature | Primer name | Primer sequence 5’-3’ | PCR product size (nt) | Amplified region |
|---|---|---|---|---|---|
|
C. labracis [JQ694148] |
66.6°C |
Caino_F |
ACGTGCAGCTCATGACACGG |
301 |
ITS1 |
| |
|
Caino_R |
TCAGTCAAGCCAGGGGAAGG |
|
|
|
M. obovata [JQ694145] |
|
Macv_F |
CCCGAGGCACTCAAAGACTG |
537 |
ITS1 |
| |
|
Macv_R |
TCAGTCGAGCCCAGGATAGG |
|
|
|
M. novaezealandensis [KJ540203] |
64°C |
Maritr_F |
TTGACATTCGGCCGGGGTGC |
214 |
ITS2 |
| |
Maritr_R |
ACCGGCCTAAAGCGCACAGA |
|
|
|
|
Philophthalmus sp. [KJ540204] |
|
Philsp_F |
CGTGAGAGATCACGCGAGG |
352 |
ITS2 |
| Philsp_R | TGTGCGCCTCACCAAGTGAG |
Species-specific ITS rDNA primers designed in this study for amplification of i) C. labracis and M. obovata, infecting G. adansonii snails from the Ebro Delta (Spain) and ii) M. novaezealandensis and Philophthalmus sp., infecting Z. subcarinatus snails from Otago Habour (New Zealand).