Table 1.
Phosphotriesterase kinetic parameters
| k cat (s −1 ) | K M (μM) | k cat /K M (M −1 .s −1 ) | |
|---|---|---|---|
|
Paraoxon |
0.12 ± 0.01 |
434 ± 54 |
2.81 (±0.38) × 102 |
|
Paraoxon 0.01% SDS |
0.28 ± 0.01 |
537 ± 48 |
5.22 (±0.51) × 102 |
|
Paraoxon 0.1% SDS |
0.25 ± 0.01 |
405 ± 21 |
6.10 (±0.34) × 102 |
|
Methyl Paraoxon |
0.31 ± 0.02 |
278 ± 40 |
1.10 (±0.17) × 103 |
|
Parathion |
ND |
ND |
ND |
|
Methyl Parathion |
ND |
ND |
4.31 ± 0.20 |
|
Malathion |
ND |
ND |
ND |
| CMP-Coumarin | 0.28 ± 0.02 | 642 ± 89 | 4.38 (±0.68) × 102 |
ND correspond to Not Detected hydrolysis. Results have been obtained with cobalt as cofactor.