Table 3.
Lactonase kinetic parameters
| k cat (s −1 ) | K M (μM) | k cat /K M (M −1 .s −1 ) | |
|---|---|---|---|
|
C4 AHL |
ND |
ND |
ND |
|
C6 AHL |
ND |
ND |
ND |
|
C8 AHL |
0.94 ± 0.02 |
178 ± 26 |
5.28 (±0.77) × 103 |
|
3-oxo C6 AHL |
ND |
ND |
ND |
|
3-oxo C8 AHL |
0.89 ± 0.07 |
836 ± 178 |
1.07 (±0.25) × 103 |
|
3-oxo C10 AHL |
1.03 ± 0.04 |
213 ± 33 |
4.88 (±0.77) × 103 |
|
γ heptanolide |
10.25 ± 0.50 |
388 ± 62 |
2.64 (±0.44) × 104 |
|
Nonanoic-γ-lactone |
2.64 ± 0.07 |
109 ± 19 |
2.44 (±0.44) × 104 |
|
Undecanoic-γ-lactone |
0.34 ± 0.01 |
578 ± 78 |
5.89 (±0.84) × 102 |
|
dodecanoic-γ-lactone |
0.53 ± 0.03 |
242 ± 60 |
2.21 (±0.57) × 103 |
|
Nonanoic-δ-lactone |
4.55 ± 0.21 |
348 ± 53 |
1.31 (±0.21) × 104 |
|
Undecanoic-δ-lactone |
1.05 ± 0.05 |
168 ± 37 |
6.22 (±1.40) × 103 |
|
Dodecanoic-δ-lactone |
3.34 ± 0.07 |
185 ± 27 |
1.81 (±0.27) × 104 |
|
ϵ caprolactone |
15.04 ± 0.47 |
1 031 ± 83 |
1.46 (±0.13) × 104 |
| Dihydrocoumarine | ND | ND | ND |
ND correspond to Not Detected hydrolysis. Results have been obtained with cobalt as cofactor.