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. 2014 Jun 24;9(6):e97946. doi: 10.1371/journal.pone.0097946

Figure 4. Increased Insulin-like Growth Factor 1 Receptor Protein and Pro-survival Signal by let7c Modulation.

Figure 4

The transfection of let7c in the PC12 cell line significantly decreased insulin like growth factor 1 receptor (IGF1R) protein (A). The western blot analysis of potential let7c targets, including IGF1R, IGF2R, and IGF2 mRNA binding protein 1 (IGF2BP1) from the hemorrhagic hemisphere was compared with the normal control hemisphere and the non-treated hemorrhagic group (B). The quantitative analysis showed that IGF1R protein was decreased by intracerebral hemorrhage (ICH), and was significantly restored by antagomir (AM) let7c treatment (C). The phosphorylated serine threonine kinase (p-Akt) was markedly increased after AM let7c (C). *P<0.05, n = 4 per group, Scr, scrambled, GAPDH, glyceraldehydes 3-phosphate dehydrogenase.