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. 2014 Jun 24;9(6):e100510. doi: 10.1371/journal.pone.0100510

Figure 2. SJ allows more efficient identification of true transformants.

Figure 2

Screen size (number of colonies on the selection plate to be examined after transformation) that would allow 95% probability of finding at least one true transformant (Kms) among revertants (Kmr) at indicated transformation frequency was calculated for Janus (solid line) and SJ (dashed line). The observed revertant frequency during transformation of Janus and SJ (Table 3) was used in screen size calculation (see Materials and Methods for calculation details).