Table 3. Comparison of false positive frequency in the Janus- and SJ-mediated negative selection.
| Recipient Strain | Donor DNA | Growth (CFU/ml) with selection on | False positive frequency | |||||
| Km | Sm | Su | Sm+Su | Selection by Sm only | Selection by Su only | Selection by Sm+Su | ||
| TIGR4J | None | 1.98×107 | 6.61×102 | NA | NA | 0.99 | NA | NA |
| 603(SmS) | 2.28×107 | 7.21×102 | NA | NA | ||||
| SpnYL001 | None | 4.85×107 | 1.80×102 | 2.70×102 | 0.3# | 0.99 | 0.50 | 0.01 |
| 603(SmS) | 5.15×107 | 1.83×102 | 5.71×102 | 27 | ||||
#
Titer below the limit of detection was denoted as one-half the detection limit.
NA: No data was collected for the TIGR4J strain when Su was used in selection because TIGR4J showed no Su sensitivity.