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. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252

Figure 1. Schematic representation of the domain structure of Flamindo2.

Figure 1

Citrine is a mutant of yellow fluorescent protein (YFP). cDNAs for yellow fluorescent cAMP indicators, Flamindo and Flamindo2, were created by insertion of DNA fragments encoding the cAMP-binding domain of mEPAC1 (199–358) and N- and C- terminus linker peptides including restriction sites. Nucleus-targeted Flamindo2 (nlsFlamindo2) was created by fusion of NLS (nuclear localization signal; MPKKKRKVEDVDP) to the N-terminus of Flamindo2.