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. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252

Figure 3. Live-cell imaging of cAMP in Flamindo2/nlsFlamindo2-expressing COS7 cells.

Figure 3

(A) Representative images showing changes in fluorescence intensity (FI) by 50 µM forskolin application in Flamindo2-expressing COS7 cells. (B) Time course of a normalized decrease in FI induced by reagents, which increase intracellular cAMP levels. Each reagent was applied at 2 min (dotted line). Three traces (black and gray lines) from single cells in three independent experiments are shown in all of the graphs. The black traces indicate representative data. (C) Representative images showing changes in FI by 10 mM bicarbonate application in nlsFlamindo2-expressing COS7 cells. (D) Different effects of bicarbonate and forskolin on cAMP dynamics in the nucleus. Bicarbonate or forskolin was applied at 2 min. Three traces (black and gray lines) from single cells in three independent experiments are shown in all of the graphs. The black trace indicates representative data.