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. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252

Figure 4. Dual-color imaging of cAMP and Ca2+ in Flamindo2 and R-GECO co-expressing HeLa cells.

Figure 4

(A) Images of changes in fluorescence of Flamindo2 and R-GECO induced by 100 µM noradrenaline. (B) Time course of changes in cAMP (solid line) and Ca2+ (dotted line) induced by 100 µM noradrenaline. Noradrenaline was applied at 120 s. Representative trace is shown in the graph.