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. 2014 Jun 24;9(6):e99958. doi: 10.1371/journal.pone.0099958

Figure 9. OGD affects the expression levels of NMDAR subunits.

Figure 9

(A) Total protein extracts were collected 7 h and 24 h after OGD and Western blot analysis was performed. Whereas total GluN1 levels tend to increase after the insult, a decrease in total GluN2A was observed after both periods of recovery while GluN2B decreased at 24 h after the OGD insult. The left panel shows a representative western blot for GluN1, GluN2A and GluN2B levels after OGD. The right panel represents the quantification of the Western blots. Actin was used as loading control. Bars represent the mean ± SEM of 5–9 independent experiments, performed in distinct preparations. (B) OGD induces a marked increase in the mRNA levels of GluN3A but not in GluN3B. Bars represent the mean ± SEM of 3–5 independent experiments, performed in distinct preparations. *p<0.05, as determined by the Student's t-test.