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. 2014 Jun 24;9(6):e100286. doi: 10.1371/journal.pone.0100286

Figure 5. Effect of urate on the subcellular distribution of Nrf2 in SY-SY5Y cells.

Figure 5

Cells were pre-incubated with urate for 30 min prior to 6-OHDA treatment for 6 h. (a) Representative pictures showing the subcellular distribution of Nrf2 (FITC/green) in SH-SY5Y cells. Nuclei were stained with DAPI (blue). Scale bar = 20 µm. (b, c) Cytoplasmic and nuclear fractions were prepared and subjected to immunoblotting analysis. H3 and β-actin were used for nuclear and cytoplasmic protein markers, respectively. Mean ± SEM, n = 4. #P<0.05 versus 6-OHDA group; ##P<0.01 versus control.