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. 2014 Jun 24;9(6):e100286. doi: 10.1371/journal.pone.0100286

Figure 6. Effects of urate on the mRNA and protein expression of the antioxidant genes.

Figure 6

Cells were pretreated with 200 µmol/l urate or vehicle, followed by 6-OHDA treatment for 14 h. The mRNA (a-d) and protein (e-h) levels of γ-gclc, γ-gclm and ho-1 were assessed by reserve transcription PCR and immunoblotting, respectively. β-actin served as loading controls. Group data were obtained by normalizing to β-actin and expressed as percentage of control values. Mean ± SEM, n = 3. *P<0.05, **P<0.01 versus control; #P<0.05, ##P<0.01, ###P<0.001 versus 6-OHDA group.