Figure 7. Nrf2-siRNA eliminated the protective effects of urate.

(a, b) SH-SY5Y cells were transfected with scrambled siRNA (si-control) or two different siRNAs targeting human Nrf2 (si-Nrf2-1 and si-Nrf2-2). RNA interference efficiency was determined by immunoblotting at 24 h later. Mean ± SEM, n = 3. *P<0.05, **P<0.01 versus control group. (c, d) Cells were exposed to 6-OHDA (50 µmol/l) for 14 h, with or without urate pretreatment at 24 h after transfection. Cell viability (c) and intracellular glutathione level (d) were measured as described above. Results were presented as mean ± SEM, n = 3. *P<0.05, **P<0.01, ***P<0.001.