A) Insulin secretion from islets exposed to 3, 8 and 16 mM glucose for 1 hour, in animals from the four different groups at the age of 17 weeks. (n≥9 experiments with 7 animals from 7 litters). The inset shows the insulin release when islets were exposed to 3 mM of glucose. B) Insulin secretion from islets exposed to 3, 8 and 16 mM of glucose for 1 hour, in animals from the four different groups at the age of 28 weeks (n≥6 experiments with ≥6 animals from ≥6 litters). C) Insulin content from isolated islets at the age of 17 weeks (n≥10 experiments with 7 animals from 7 litters). D) Insulin content from isolated islets at 28 weeks (n≥8 experiments with ≥6 animals from ≥6 litters). Data are expressed in mean±SEM, p<0.05 by one way ANOVA followed by Tukey, Dunnet or Dunn's method; a control vs. BPA; b control vs. HFD, c control vs. HFD-BPA, d HFD vs. BPA, e HFD vs. HFD-BPA, f BPA vs. HFD-BPA.