Figure 4. Production of IFN-γ by mononuclear cells from immunized mice.

Proliferative responses of mononuclear cells obtained from mice immunized with eight synthetic peptides, and further in vitro-stimulated with 10 µg/mL of corresponding coiled coil peptides. Conconavalin A (Con A) mitogen was used as a positive control. RPMI 1640 medium was used as a negative control. Data of SFC were reported as mean ± SEM for each peptide. P value was calculated by the ANOVA test. MN: mononuclear cells.