Figure 5. Schematic representation of the antigen selection process.

Download selection is represented: first, 50 P. vivax antigens containing α-helical coiled coil motifs, selected from orthologues of P. falciparum, were chemically synthesized and tested with PNG sera. Seventeen reactive with prevalence >30% were tested with Colombian sera. Eight antigens with prevalence >50% either with PNG or Colombian sera were used for mice immunization and immune response testing. Four antigens were finally selected for further pre-clinical testing.