Skip to main content
. 2014 Jun 24;9(6):e100787. doi: 10.1371/journal.pone.0100787

Figure 4. UPR participated in the up-regulation of A20.

Figure 4

(A) Cells were treated with tunicamycin (TU; 2 µg/ml), pORF3, or were untreated (UN) for 48 h, and subjected to western blotting. (B) Spliced ATF6 was detected in A549 cells with or without pORF3 pretreated by western blotting. (C) A549 cells were pretreated with (+) or without (−) 250 µM AEBSF for 1 h and then transfected with pORF3 or not. Protein extracts were processed for western blotting by using A20 antibody. (D) Cells were co-transfected with pNF-κB-Luc and pORF3 for 48 h, and the other group that was transfected only with pNF-κB-Luc served as a control. Both groups were exposed to TNF-α for 6 h and subjected to a luciferase assay. Another group was exposed to AEBSF (250 µM) before co-transfection with pNF-κB-Luc and pORF3. Luciferase activity was normalized to β-galactosidase, and fold changes against the control are presented. The results are representative of three independent experiments (each performed in triplicate). *P<0.05.