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. 2014 Jun 18;9:2993–3003. doi: 10.2147/IJN.S61103

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© 2014 Yao et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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(A) Confocal image of HEK293 cells after incubation with RITC labeled CSO-SA/pDNA and CA-CSO-SA/pDNA nanocomplexes (red) for 4 and 24 hours at 37°C, cell nucleus (blue) was stained by Hoechst33342. (B) The quantitative analysis of cellular uptake of RITC labeled CSO-SA/pDNA and CA-CSO-SA/pDNA nanocomplexes, intracellular fluorescence intensities was determined by flow cytometry.

Abbreviations: CA, cis-aconitate; SA, stearic acid; CSO, chitosan oligosaccharide; pDNA, plasmid DNA; RITC, Rhodamine B isothiocyanate.