Skip to main content
. 2014 Jun 24;9(6):e100948. doi: 10.1371/journal.pone.0100948

Figure 1. Schematic illustration of the HVAS vector system and its assembly process.

Figure 1

1A, Assembly of a functional module in pBASE vectors from elements in pENTR vectors; 1B, Assembly of multi-modular construct in pHR assembly vectors from modules in pBASE shuttle vectors. Up to 4 elements in pENTR vectors can be assembled on each pBASE shuttle vector, and up to 4 modules in the pBASE shuttle vectors can sequentially dock onto the pHR assembly vector. Abbreviations: L1, attL1; R5, attR5; L5, attL5, L4, attL4; R4, attR4; R3, attR3; L3, attL3; L2, attL2; R2, attR2; R1, attR1; HE, homing endonuclease; DEST, destination cassette; HE1, I-SceI; HE2, I-CeuI; HE3, PI-SceI; HE4, PI-PspI; ITR, inverted terminal repeat.