Figure 2. Building of a functional two-module construct with HVAS which supported doxycycline-induced GFP expression.

2A, schematic illustration of the construct. The abbreviations of depicted elements were annotated in Table 1. 2B, above construct was stably transfected into HCT116 cells, and selected with 1 µg/ml puromycin. Surviving cells were treated with or without 1 µg/ml doxycycline over night before image acquisition with bright field and fluorescent microscopy.