Figure 6. A long-lived inflammatory state is induced in murine skin after 2 Gy of gamma radiation.

Total RNA from murine skin prior to and at the indicated time points after gamma radiation (2 Gy) were obtained. Quantitative reverse transcriptase PCR arrays were performed. Three to 6 mice per time point were analyzed. All samples demonstrated no DNA contamination or inhibitors of PCR and were first normalized for housekeeping genes, averaged for each time, and expressed as relative change versus untreated. P-values were calculated using the Student’s t-test of the replicate 2∧(−Delta Ct) values for each gene in the control versus treatment groups. The calculation of p-values for each gene in general found significance below 0.001 for changes greater than 2-fold.