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. Author manuscript; available in PMC: 2014 Jun 24.
Published in final edited form as: Carcinogenesis. 2006 Mar 14;27(9):1748–1757. doi: 10.1093/carcin/bgl005

Fig. 5.

Fig. 5

HYD1 interacts with α6 and α3 integrins. Affinity precipitation reactions were performed with HYD1, RZ3 or HYDS peptides on DU-145H cell membrane fractions as described in Materials and methods. The precipitates were analyzed for α6, α3 or α2 integrin by SDS–PAGE electrophoresis followed by a western blot with AA6A antibody (α6 specific), Antibody 1920 (α3 specific or α2 specific). Mb (membrane lysate) indicates the starting material. Depletion of the membrane lysate of β1 containing integrins by TS216 (β1 integrin specific) will retrieve the α6 integrin. No α6 integrin can be recovered by HYD1 if the lysate has been pre-cleared of β1 containing integrin (HYD1 post TS2/16).