Fig. 5.

The SIMS imaging analysis of a multinucleated giant glioblastoma cell from a 2 hr treatment with 110 μg/g boron equivalent of ¹⁰BPA. SIMS images revealing the subcellular distribution of designated isotopes are shown in panels A-E. The ¹⁰B image (D) is shown in red and the ¹²C image in green (E). The overlay image of ¹⁰B/¹²C illustrates boron gradients in yellow, via mixing of red and green, in the giant cell (F). The position of 4 nuclei, designated as N1-N4, and the perinuclear region (PNC) in the giant cell is illustrated in the ⁴⁰Ca (C) and the overlay image panel (F). The image integration times for ³⁹K and ²³Na images were 0.4 s. The ⁴⁰Ca, ¹²C, and ¹⁰B images were integrated for 2 min each.