Fig. 1.

a Summary of known and novel candidate TPD52 partners identified. (+), growth on solid SD/-His–Leu–Trp media at 30 °C after 5–6 days; (++), after 3–4 days; (+++), after 1–2 days; (−), no visible growth after at least 7 days; ND not done. Results were obtained from three independent experiments in which each interaction was assessed in triplicate. b Hf7c cells co-transformed with PLP2 or RAB5C pAD-GAL4 prey vectors and pAS2-1TPD52 bait, or empty pAS2-1 vector. Triplicate colonies were transferred to solid SD/-His–Leu–Trp media, and incubated at 30 °C for 9 days, demonstrating growth of TPD52 and PLP2 or RAB5C co-transformants, but not of bait vector and PLP2 or RAB5C co-transformants. c Proportions of known partners (dark grey), candidate novel partners (black) and false positives (light grey) identified according to Y2H screening duration. Known partners were most frequently identified by 5 days of screening (<1 week), novel partners were most frequently identified at 1 week (1 week), and false positives were the most frequent prey category at 1 week, and between 1 and 2 weeks of screening (>1 week)