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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Clin Exp Allergy. 2014 Jul;44(7):986–998. doi: 10.1111/cea.12324

Figure 3.

Figure 3

Minimally cross-reactive CD4+ T-cells can be detected ex vivo in TGP allergic subjects. Determination of frequencies of Phl p- and grass homolog-reacting CD4+ T-cells for different regions of Group 1 and 5a allergens. A. Example of ex-vivo tetramer staining for TGP–derived Group 1 97-116, Group 1 221-240 and Group 5a 97-116-Phl p-specific and homologous minimally cross-reactive CD4+ T-cells from a grass pollen allergic subject with an ImmunoCAP score of 5 for Phl p specific-IgE. B. Frequencies for TGP-derived Group 1 97-116 and Group 1 221-240, and Group 5a 199-218 grass homolog-reactive CD4+ T-cells. Each data point denotes the frequency of epitope specific CD4+ T-cells for a different individual. A t student test was used in the statistical analysis.*P < 0.05 to compare populations from different epitopes.