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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Clin Exp Allergy. 2014 Jul;44(7):986–998. doi: 10.1111/cea.12324

Figure 5.

Figure 5

Minimally cross-reactive CD4+ T-cells functional profiles correlate with a TH2 dominated response. An example of intracellular cytokine staining for Phl p- and grass homolog-reactive T-cells is provided. A. Representative result for ICS. PBMC from a TGP allergic subject were stimulated with Poa p peptide corresponding to region Group 1 97-116 and cultured for 2 weeks, cells were than stained with the corresponding PE-labeled DR04:01/Poa p grass peptide-loaded tetramers. Tetramer+ cells were gated and dual cytokine analysis is presented. B. Summarized results of ICS for TGP-derived Group 1 97-116, Group 1 221-240 and Group 5 199-218 epitopes, each bar depicts percentage of tetramer-positive cytokine producing cells in each individual.