Table 1.

Phl p and grass homolog CD4+ T-cell epitopes

Group allergen a	Grass species b	Aminoacid sequence c	IC50 (μM) d	RBA e
Group 1 0102 97-116	Phl p 1	EEPIAPYHFDLSGHAFGAMA	0.274590811	1
	Lol p 1	EEPIAPYHFDLSGHAFGSMA	0.435355464	0.63072784
	Pha a 1	EEPIAPYHFDLSGHAFGSMA	0.435355464	0.63072784
	Poa p 1	EEPIAAYHFDLSGKAFGAMA	0.826658242	0.33216969
Group 1 0102 221-240	Phl p 1	TEAEDVIPEGWKADTSYESK	2.921366199	1
	Lol p 1	SEFEDVIPEGWKADTSYSAK	2.921366199	1
	Poa p 1	GEAEDVIPEGWKADTAYASK	0.453158364	6.44667832
	Dac g 1	SEVEDVIPEGWKADTSYEAK	0.176697335	16.533165
Group 5 01 01 167-186	Phl p 5a	AAFKVAATAANAA	0.215901172	1
	Hor v 5a	AAFRTAATAADAA	0.970389302	0.22248923
	Hol l 5a	AAFRIAATAANAA	0.116004811	1.86113982
	Lol p 5a	AAYRTAATAANAA	0.531949004	0.40586818
	Pha a 5a	AAFKIAATAANSA	0.38603706	0.55927576
	Poa p 5a	AAFKVAATAANAA	0.05	4.31802348
	Dac g 5a	AAYKIAATAANAA	0.05	4.31802348
Group 5 0101 199-218	Phl p 5a	ESYKFIPALEAAV	0.970389302	1
	Hor v 5a	ESYKFIPALEAAV	0.990031471	0.98016006
	Hol l 5a	EAYKFIPSLETAV	0.104945543	9.24659849
	Lol p 5a	DSYKFIPTLVAAV	0.409955767	2.36705855
	Pha a 5a	ETYKFIPSLEAAV	0.051012076	19.0227368
	Poa p 5a	DTYKSIPSLEAAV	0.061093499	15.8836753
	Dac g 5a	ESYKFIPTLEAAV	0.074648485	12.9994507

^aTwo distinct regions were used for Group 1 (20-mer) and Group 5a (13-mer) major allergens; Major allergen Phl p 5 consists of two isoforms, Phl p 5a and 5b.

^bPeptides for different grass species were used.

^cHomologous sequences of amino acids for each epitope region. Bold letters variant residues. Underlined amino acids depict DRB1*0401 motifs P1, P4, P6, P7 and P9.

^dIC50, concentration required by a target peptide to inhibit 50 % binding of the reference peptide.

^eRBA, relative binding affinity (relative to that of the Phl p peptide).