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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Genesis. 2014 May 8;52(6):636–655. doi: 10.1002/dvg.22785

FIG. 5. Habenular connectivity with the IPN revealed by transgenic reporters.

FIG. 5

gng8:GFP labeled habenular axons terminate in discrete, stereotypic bundles in the dorsal (D), intermediate (I) and ventral (V) IPN of (a, d) adult and (g, j) larval brains. (b, e) In the adult brain, brn3a:GFP labeled dorsal habenular neurons predominantly contribute to the D-ii, intermediate and V-i and V-iii bundles and (c, f) nptx2:Gal4;UAS:DsRed2 labeled axons terminate in the D-i, D-ii, intermediate and V-ii IPN bundles. (h, k) In the 4 dpf larval brain, IPN innervation patterns are similar to the adult when the UAS:DsRed2 reporter is used. (i, l) However, with Tg(4xUAS:GFP) regulated by the nptx2:Gal4 driver, labeled fibers preferentially innervate the dorsal and intermediate IPN. Scale bar is 100 μm for a–f and 30 μm for g–l.